1.E4/E6 ratios of HA firstly inc
HA的E4ffi6比值也提高，随培养时间的延长，HA的E4／E6比值先提高而后下降，转折点是33天。收藏指正
2.Beijing A-Z TOWN(A5-9 Zone)
北京A-Z TOWN(A5-9区)收藏指正
3.SDS PAGE with silver staining showed about 85% purity of the isolated F o and 9 different subunits including b, OSCP, d, a, e, F 6, IF 1, A6L and c.
SDS 聚丙烯酰胺凝胶电泳鉴定表明 ,纯化的Fo 含有b、OSCP (寡霉素敏感授予蛋白 )、d、a、e、F6、IF1、A6L和c等 9种亚基 .收藏指正
4.Results:The recombinant plasmid pUC18 E6 had been got.
结果 :获得重组质粒 pUC18 E6。收藏指正
5.Methods Plasmids containing HPV-16 E6 or E7 were used to generate absolute standard curves.
方法将制备的含HPV-16E6和E7基因的质粒作为标准品,建立实时荧光定量PCR和RT-PCR方法,分别对3种宫颈癌细胞株进行HPV-16E6和E7基因的DNA和RNA拷贝数的定量检测。收藏指正
6.However, the expression level of wild type HPV16 E6/E7 fusion protein was very low, and the in vivo antitumor efficacy of the HPV16 E6/E7 fusion protein has not
为了提高E6／E7融合蛋白的表达水平并进一步提高其免疫原性，我们对野生型HPV16E6／E7融合基因进行了改造，构建了HPV16 mE6Δ／mE7Δ和mE6Δ／mE7两个融合基因，同时引入了3个点突变，并在原核系统中进行表达。 结果收藏指正
7.Chromosomal Aberration and HPV18 E6,EPV18 E7 Expression in Esophageal Epithelial Cells during Immortalization and Malignant Transformation
食管上皮细胞永生化及恶变中染色体异常和HPV18E6、HPV18E7表达收藏指正
8.THE EFFECT OF MONOCLONAL ANTIBODY 2E6 ON THE ACETYLCHOLINESTERASE ACTIVITY
单克隆抗体2E6对乙酰胆碱酯酶活性的影响收藏指正
9.Conclusion Cellular immunity in mice could be enhanced induced by DNA vaccines based on HPV16 E6/E7. It could be much stronger induced by DNA recombinants of HSP70 of mycobacterium tuberculosis and HPV16 E6/E7. However, humoral immunity remained.
与结核杆菌HSP70重组后,IL-10及抗体水平没有明显变化。 结论以HPV16 E6/E7为基础的DNA免疫能增强小鼠的细胞免疫反应。收藏指正
10.Specific Suppression of HPV18 E6 Oncoprotein by RNA Interference in Hela Cells
RNA干扰技术特异抑制HPV18E6基因表达的研究收藏指正