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1.After the purification of the organisms, the cul tures of all the ten strains were grown in 100 ml of trypticase soy broth T. S. B. in the 250 Erlenmeyer Flask at 37℃ with shaking for 3 days. Then they were centrifuged at 4500 rpm and the supernatants were assayed by radioimmunossay (RIA) for the beta—hCG subnit.
这些细菌按照常规的细菌学鉴定法鉴定,纯和鉴定后,每一株茵培养在含100ml大豆胰蛋白胨液的三角中,置37℃下振荡培养(180rpm)3天,培养液以4500转/分离心15分钟,去除菌体,上清液用放射免疫法测定,其中有八株细茵能产生hCG类似物。收藏指正
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