包装法
1.He also devised a new packaging system for preserving coffee in which the cans were filled with inert gases instead of air: pressurization.
2.The paper canvasses the application of EPR, including container deposit legislation. The National Packaging Covenant, a form of product stewardship, is also extensively discussed.
3.A packaging engineer from Hamburg designed this so-called tango pack, using much less cardboard than usual. The cans are held firmly together by a narrow plastic trip.
4.The recombinant lentiviral Lent/NT4-NAP was packaged by calcium phosphate precipitation.
5.Methods Co-transfect virus packaging cells(293 cells)with rAAV vector system by calcium phosphate precipitation to synthesize rAAV-HPV16E7AS and rAAV-lacZ respectively. Determine the titers of recombinant viruses by dot hybridization. Transfect 293 cells with the recombinant viruses and determine the transfection efficiency.
6.Methods: Retrovirus was packaged in vitro with PA317 cells and Jurkat cell was transducted with collected virus; The expressions of bcl 2 mRNA and protein were assayed by RT PCR and Western blotting, respectively.
7.Methods:Drug packaging materials were analyzed by ATR (Attenuated Total Reflectance) infrared spectrophotometry and transmittance infrared spectrophotometry.
8.Renalembryo 293 cell was co-transfected with the rAAV vector of plasmid pSSHG/NT4-GFP-Ant ,packaging plasmid pAAV/ Ad and helper adeno virus pasmidpFG140.The recombinant AAV viral stock was titrated by dot blot.
9.2. To genenatethe pAAV-LacZ viral particles, using LacZ as a report gene, AAV-293 cells wasco-transfected with the three plasmids(pAAV-LacZ, pAAV-RC, and pHelper) bythe way of liposome-mediated transfection.
10.Methods: The TK gene was transfected into human gastric carcinoma cell line MKN28 using HSV TK that packed with PA317 cell, the sensitivity of MKN28TK cells to GCV was examined in vitro .
