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1.Florfenicol has been demonstrated to be active in vitro and in vivo against Mannheimia (Pasteurella ) haemolytica, Pasteurella multocida, and Haemophilus somnus.
氟苯尼考在体内、体外均有明显活性,能够抑制溶血曼海姆、多杀巴斯德和睡眠嗜血杆收藏指正
2.Lambs in group A (N=13) were inoculated with both agents, group B (N=4) with RTS-42 followed by sterile broth, and group C (N=4) with sterile cell culture fluid followed by broth containing P. haemolytica.
A组(13头)羔羊以腺病毒与巴斯德相继感染,B组(4头)以RTS—42株感染继之以无肉汤注射,C组(4头)注射以无细胞培养液继之以巴斯德的肉汤培养物感染。收藏指正
3.Four groups of 5- to 10-day old colostrum-deprived lambs were used to evaluate the response to ovine adenovirus (OAV) strain RTS-42 followed by Pasteurella haemolytica (type 1A) 5 days later.
4组5至10日龄禁食初乳的羔羊,用于评价对绵羊腺病毒RTS—42株与5天后继之以溶血性巴斯德(1A型)感染的反应。收藏指正
4.Cultured sour cream is homogenized pasteurized cream that is soured with streptococcus lactis at 71?(22℃) until the level of acidity reacses at least 0.5%.
人工养殖酸化的酸奶油中的油脂粒均匀分布,巴斯德消毒后的奶油,用乳酸链球产酸,温度在71?(22℃),直到酸度至少抵达0.5%。收藏指正
5.According to the partiality codon of Pichia pastoris, antibacterial peptides cecropin B gene was reconstructed, synthesized and cloned into pPIC9K to construct the recombinant expression vector CB-pPIC9K. The CB-pPIC9K was transformed into P. pastoris KM71. Under the control of the promoter AOX, cecropinB protein was expressed with the high level of 200μg/mL in the flask-shaking culture fermentation. In addition, the cecropinB protein with antibacterial activity was analyzed.
根据毕赤氏巴斯德酵母(Pichia pastoris)偏好密码子,改造并化学合成天蚕素基因B,克隆到 pPIC9K载体中,构建分泌型重组酵母表达载体CB-pPIC9K,转化Pichia pastoris受体KM71,在醇氧化酶(AOX) 启动子调控下,Cecropin B获得表达,分子量约3.8KD,摇瓶发酵产率可达到200μg/mL,并对其抗活性进行了初步测定。收藏指正
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