确证物
1.At present, about 15 human UGT have been identified, and muc h more knowledge about substrate selectivity of UGT has been reported. However, the study on enzymology of UGT lags behind that of cytochrome P450.
2.The structures of FcCH=CH-CH=CH-OCH and FcCH=CH-CH=CH-CH=CH-RuCl(CO)(PMe3)3 have been confirmed by X-ray diffraction.
3.AIM To isolate and identify a glucuronide metabolite of SFZ 47 [3H 1,2 dihydro 2 (4 methyl phenylamino)methyl 1 pyrrolizinone], which is difficult to synthesize because it undergoes hydrolysis and intramolecular acyl migration at physiological pH, in rabbit urine.
4.RESULTS The target compound was provided through a five-step procedure starting from maleic anhydride with corresponding total yields of 31%,24%and 35% respectively. The structure was verified by ~ 1 H-NMR and HRMS.
5.By Confocal Laser Scanning Microscopy(CLSM) and combined with fluorescence probe, 457Da Lucifer Yellow (LYCH)which was impermeable to the membrane, could enter the living parenchyma cell through ectodesmata-like. The result proved that ectodesmata-like still retained the function of the symplastic translocation.
6.2、Two estron derivatives and two isoflavone derivatives were synthesized and their structures were confirmed by UV, ~1HNMR.
7.The binaphthols compounds 1a~1b were biomimetic oxidated to afford novel structure dibenzoxanthenes 2a~2b with the complex of CuCl2·2H2O-amine to mimic oxidoreductase. The product structures were characterized by 1H NMR,IR spectra and ESI-MS.
8.The effects of reaction medium,reaction temperature and reaction time on the yield of the product CMESA were studied. The chemical structure of the product was identified by elemental analysis,functional group analysis and IR spectrum. The optimal conditions for the synthesis are,reaction time,16 h,reaction temperature,85 ℃,molar ratio of GTMAC to stearic acid,1∶1,reaction medium,isoproanol,product yield,86.8 %.
9.A series of α aminoalkylphosphonate derivatives of norcantharidin, 5,6 dehydro norcantharidin and exo bicyclo 2.2.1 hept 5 ene 2,3 dicarboxylic anhydride were synthesized by the condensation of α aminoalkylphosphonic diphenyl ester with norcantharidin, 5,6 dehydro norcantharidin and exo bicyclo 2.2.1 hept 5 ene 2,3 dicarboxylic anhydride, and their structures were confirmed by 1H NMR, MS, IR and elemental analysis.
10.The effects of reaction medium,reaction time and amounts of acid acceptor pyridine on the yield of the product CDESA were studied and its chemical constitution was proved by elementary analysis,ester value,hydroxyl value and IR spectrum. Thus,the reaction was conducted with n(CMESA)∶n(stearyl chloride)∶n(pyridine)=1∶1∶1 in 1,4-dioxan under reflux for 3 h to give CDESA in 93.1% yield.
