293t
1.RESULTS :The three plasmids were effectively transferred into 293T c ells.
2.of 3.The expression of GFP in mouse T lymphocytes was observed by fluorescence microscopy and fluorescence-activated cell sorting(FACS). The results showed that the transfection efficacy was 63.04±7.24% in 293T cells analysed by FACS and the viral titer was(3.09±0.61)×106 U/ml.
3.Purpose To isolate the full-length human BMP2 cDNA and construct the recombinant lentivirus DNA pHIV-CS-CDF-CG-PRE-hBMP,the recombinant lentivirus was packaged and grew in 293T cells DNA.
4.Plasmids of the pCMV5-AML1/AML1-ETO and the MDKp-pGL3-B or mu-MDKp-pGL3-B were then co-transduced into 293T cells and the luciferase relative value was analyzed.
5.OBJECTIVE:To construct the vectors, pDsVEGF165Red1-N1 and pIRES2-BMP2-enhanced green fluorescent protein(EGFP) followed by co-transfected into HEK 293-T cells,and study their expression and location of VEGF165 and BMP2 in the cells.
6.Methods:The 293T cells was cotransfected with PDG and an AAV plasmid containing human apoAIcDNA to produce infectious rAAV, and it was purified by non ionic iodixanol gradients followed by heparin affinity chromatography.
7.Similar phenomena were demonstrated in 293T and U373-MAGI-CCR5e cells. Additionally, our results indicated that the inhibition of cyclin Tl expression by shRNAs was both dose- and time-dependent. CDK9/cyclin T directs its activity in a cell cycle independent manner and was involved in transcription during the elongation steps.
8.Objective To construct the mutants of the four serine phosphorylation sites(104,106,118 and 167) in ERα AF-1 and detect their effects on the transcriptional activity of Erα in 293T cells.
9.Methods The replication-competent HBV recombinant plasmid pHBV4.1 plus different liver-enriched transcription factor (HNF1,HNF3,HNF4,HNF6,C/EBP and RXRα/PPARα) expression plasmids were co-transfected into nonhepatic cell lines (NIH3T3,HeLa,293T,SW1353,CV-1 and COS1).
10.Results 50 nmol/L siRNA-HPV-6bE7 elicited the highest level of gene si- lence in B16 which were transfected with siRNA after 48 h(87.05%),Comparing to 293T,the highest gene si- lence was caused by 10 nmol/L(78.87%).
