C terminus
2.It encodes a BTB/POZ domain at the N-terminus and a PCNA binding motif (QTKV-EFP) at the C-terminus.
3.DOC-2 has a phosphotyrosine interactingdomain (PID) on in its N-terminus and multiple proline-rich SH3 binding motifs in its C- terminus.
5.CEMA consists of eight amino acid from N-terminus of cecropin A and C-terminus of modified melittin. It maintains potent antibacterial activity and deprives of the hemolytic activity.
6.dehydroquinate dehydratase was a(α2/β2)3 peptide,of which a pair of anti-parallel β-strands existed in the N-terminus and a loop in the C-terminus;
8.Methods:Plasmids pEZ1 (Wt PgFtsZ), pYW1(ZΔC01, missing 73 residues from the C-terminus of PgFtsZ) and pYW2 (ZΔC02, missing 128 residues from the C-terminus of PgFtsZ) were introduced into Escherichia coli BL21(DE3)pLysS to express and purify the purpose proteins.
9.CaCl2, Urea, TCA can release 33 kD protein, because they may alter the C-terminus conformation of the 33 kD protein.
10.The cellular location and topology of 7a were determined by immunocytochemistry and we found that 7a protein was located at Golgi, with its N-terminus facing the cytoplasm and its C-terminus facing Golgi matrix.
