Chrysanthemum L.
1.Spontaneous hypertensive rats (SHR) were put in a special box containing fumigant of chrysanthemum indicum L. Co. Heat the box so that the temperature in it could be kept at 39.5℃～41.0℃ for 60 minutes. Then take out the rats and meassure their blood pressure.
2.The shoot tips with a length of 0.5~0.8 mm is suitable for explants,which came from Chrysanthemum with CMV.Callus grew well on MS+BA2.0 mg/L+NAA0.1 mg/L and 96.0% explants formed.
3.Diamonsil~ TMC_ 18(4.6mm×150mm,5μm) as colum(n),0.1mol·L~ -1 NaH_2PO_4-CH_3OH(7.3∶ 2.7)as mobile phase,at wavelengh of 326nm,the content of chlorogenic acid in Sichuan chrysanthemum was separed and determined by HPLC.
