DNA homology
1.Assay of Genomic DNA Homology Among & Candida Species by Total Chromosome DNA Hybridization
2.ETERMINATION OF DNA G+C mol% AND DNA HOMOLOGY BETWEEN EXTRA-SLOW-GROWING SOYBEAN RHIZOBIA AND OTHER RHIZOBIA
3.The DNA G+C mol% for extra-slow-growing soybean rhizobia (ESG) and DNA homology for ESG strains and other rhizobia were carried out by thermal denatu-ration temperature (Tm) and reassociation rate of DNA, respectively.
4.The SDS-PAGE electropheresis of whole-cell proteins was applied in classification of 71 strains isolated from Astragalus spp..It was showed that the technique is a simple and rapid method in classification of rhizobia.The similarity of strains in the same group is 78%,and DNA homology is above 70%.
5.soja was 59. 2-63. 5% and the DNA homology among five representative strains of ESG was greater then 7 0 % ( range ,72. 1 to 93. 4%), which indicated that these strains were in one genetic group. (2) DNAs from 5 ESG representative strains had very low mean homology (range,3. 8 to 30. 0%) with 16 other reference rhizobial strains.
7.But the comparison amino acid sequence shows that GPV DNA has high homology with the human adeno-associated virus AAV-2 in dependovirus and B19 in erythrovirus.
8.In the 1.2 kb fragment, there were also several conserved motifs such as CAAT-box and GAGA-box, which are related to regulation of transcription, and the putative binding sites of transcriptional factors such as EBP, EFII, NF-E1and LV. The results of BLAST showed that the DNA sequence shared high homology with the 5′-upstream region of Dunaliella viridis NR genome.
9.Y4 fragment was cloned into a PMD18-T vector and the nucleotide sequence determined. Partial sequencing of the CPV-VP2-Y3? Y4 DNA fragment showed 99% nucleotide homology with that of the canine parvovirus,strain CPV/HN-1.
10.The DNA sequence of the cloned NS1 gene showed a 99%homology with the DEN2 NGC(Genbank:AF038403).
