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1.Molecular Genetic Analysis of an Unusual DNA Modification in Streptomyces Lividans
变铅青链霉菌DNA异常修饰系统的分子生物学研究收藏指正
2.A novel DNA modification discovered in Streptomyces lividans is different from DNA methylation. This unusual modification causes wild type S. lividans DNA sensitive to site-specific oxidative double-strand cleavage (Dnd phenotype, DNA degradation).
变铅青链霉菌的DNA异常修饰系统是一种不同于甲基化修饰的新型修饰系统,它可使变铅青链霉菌DNA在电泳时易遭到氧化双链切割(Dnd表型,DNA degradation)(Zhou et al.,1988)。收藏指正
3.A novel DNA modification discovered in Streptomyces lividans 1326 is different from DNA methylation. This unusual modification causes wild type S. lividans 1326 DNA sensitive to site-specific oxidative double-strand cleavage (Dnd phenotype).
野生型变铅青链霉菌具有一种不同于甲基化修饰的新型DNA硫修饰系统,使DNA在电泳时易遭到氧化双链切割而导致降解(Dnd, DNA degradation)表型(Zhou et al., 1988, 1994, 1999)。收藏指正
4.Preliminary study revealed that such DNA modification involves incorporation of sulphur. The entire functional dnd cluster, 8.3kb in size, including 3 ORFs-dndA, dndB and dndC was involved in this DNA modification.
己证实该菌株染色体上一段8.3kb的区域与Dnd表型有关,序列分析显示该区域包含了3个ORFs—dndA, dndB和dndC(Zhou et al., 1999;李爱英,2000)。收藏指正
5.Isolation and Characterization of Genes Encoding Histone Modification Enzyme and DNA Methyltransferase in Wheat (Triticum Aestivum.L)
小麦组蛋白修饰酶基因及DNA甲基转移酶基因的分离及鉴定收藏指正
6.(1) we discuss Factors of genomic DNA degradation in Braconidae specimen. These factors include high temperature, oxidation and chemic modification. We should douse Braconidae specimen in 100% ethanol with 50mmol/L EDTA.
1.本文探讨了高温,氧化,化学修饰等影响茧蜂标本基因组DNA降解的因素,对室内保存寄生蜂标本提出应用含50mmol/L乙二胺四乙酸(EDTA)的无水乙醇固定,并在液面覆盖石蜡油等可行性建议。收藏指正
7.Studies on Fabrication and Surface Modification of Thermoplastic Microfludic Chips and Applications in Chip-based DNA Analysis
热塑性聚合物微流控芯片制作、表面改性及在DNA分析应用中的研究收藏指正
8.coli were tested for developing an conjugation system for S. nanchangensis NS3226.A dnd gene cluster, which encodes an unknown modification system for 5 Hvidans 1326 and renders its DNA susceptible to site-specific double-strand DNA cleavage during electrophoresis was conjugated from E. coli into S. nanchangensis NS3226. The total DNA of exoconjugants acquired DNA degradation phenotype during electrophoresis, which suggested that the dnd gene cluster was heterologously expressed.
将克隆在整合型载体pSET152上的变铅青链霉菌1326的dnd基因簇通过接合转移导入野生型南昌链霉菌NS3226中进行异源表达,观察到接合转移子的DNA获得了在含Fe~(2+)的电泳缓冲液中电泳时降解的表型。收藏指正
9.A technique based on a recent modification of the polyerase chain reaction (PCR) was used to amplify random sequences from one pair of wheat near-isogenic lines that differed for a powdery mildew resistance gene(Pm4a) in order to identify the randomly amplified polymorphic DNA(RAPD) markers linked to the gene for resistance to powdery mildew.
以10个核苷酸长的随机引物,通过多聚酶链式反应(PCR)技术对1对小麦抗白粉病近等基因系的基因组DNA进行了扩增,旨在鉴定出与抗白粉病基因连锁的分子标记。收藏指正
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