FACS assay
1.Its antigen activity was assayed by Western blotting, and its ability to induce the proliferation of CD4+ cell was confirmed by FACS assay.
2.In addition,the peritoneal cells were stained with PE-CD11c antibody,then stained with rabbit anti-MIP-1α and FITC labeled anti-rabbit IgG,followed by FACS assay for cell surface marker CD11c and intracellular MIP-1α.
3.then these cells were subjected to FACS assay to detect the cells appeared in upper left quandrant in dot plot. this group is the CD8 + cells with reduced CFSE and stand for proliferated CD8 + lymphocytes (CD8 +CFSE low ).
4.And used MTT assay, ELBA, FACS to value the effects of BMSC on the murine immunocompetent cells.
5.Surface marker assay by FACS showed that DCs expressed CDla (55.53%), CD80(83.78%), DR (98.76%), CD86( 99.08%), CD83 (64.70%) and CD40(76.23%).
6.TUNEL assay showed that treated cells were in the course of apoptosis, and FACS analysis showed that the apoptotic rate reached up to 42.58%.
7.Finally,BALB/c mice were vaccinated with the recombinant. Anti-EEEV antibodies from immunized mice sera were detected by immunofluorescence assay and ratio of CD4+ to CD8+ T lymphocytes was measured by FACS.
8.Surface marker assay by FACS showed that DCs expressed CD83(94.2%), which is the relative specific marker of DCs, andHLA-ABC (98.3%), HLA-DR (96%), CD4O (97.7%) and CD8O(98.2%).
9.Methods: SK-N-SH cells were pretreated using 300 μM CoCl2 for 3 h, then 24 hours later the cells were treated with 30? g/ml BCNU. MTT assay was used to detect cell proliferation and FACS was used to determine the apoptotic index.
10.METHODS After liver lavement, the hepatic lymphocytes of C57BL/6 mice were isolated and the NK cells were purified and cultured with different concentration MPA for 24 h. Then NK cells cytotoxicity was determined by [3H]TdR release assay, the secretions of IFN-γ,IL-2 and IL-10 were measured by ELISA, and the expression of NK cell receptor NKG2D,NKG2A and Ly49A was measured by FACS.
