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1.Gastric inhibitory polypeptide (GIP); Galanin (GAL); Fructose-1,6-bisphosphatase (FBP1);
2.采用电脑克隆策略、RT-PCR和RACE技术,获得了CCK、CPB1、FBP1、GIP基因的全长cDNA序列,并推导出了相应的氨基酸序列。收藏指正
2.As a preliminary stage of this study, three genes coding for three important Calvin cycle enzymes, i.e. triosephosphate isomerase (TPI), fructose 1,6 bisphosphate aldolase (FBP aldolase), and fructose 1, 6 bisphosphatase (FBPase), respectively, have been cloned into one plasmid, pTrcFAT, which is controlled by promoter trc . Successful co transcriptional expression of these three genes resulted in high yields of these enzymes under the induction of 0.25 mmol/L IPTG.
作为研究的初始阶段 ,将编码丙糖磷酸异构酶、果糖 1,6 二磷酸醛缩酶及果糖 1,6 二磷酸酶的 3个基因构建进一个由启动子trc控制的表达质粒 pTrcFAT ,成功地在大肠杆菌中实现了上述 3个基因的活性共表达。收藏指正
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