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1.Comparison of NPC Transforming Gene Tx to Ig Kappa Constant Region Gene and Their Expressions in Different Cell Lines
Tx基因与IgK基因的同源性研究及其在不同细胞株的表达收藏指正
2.The 2 8 kb Eco RⅠ/ Eco RⅠ fragment of Tx gene showed that it was highly homologous with Ig kappa constant region. Based on the restriction map of Tx gene,another Xho Ⅰ/ Eco RⅠ Tx 3 0 fragment was sequenced and analyzed by bioinformatics.
在对其中 2 .8kb片段测序的基础上 ,对其中 Xho /Eco R 长度约 3.0 kb的片段 ( Tx3.0 )进一步进行了测序 ,并利用生物信息学技术分析认为 ,Tx3.0与人类免疫球蛋白 kappa( Igκ)轻链基因高度同源 ,并直接映射于 J区 .收藏指正
3.The extramembrane region consists of 758 aminoacids, including signal peptide containing 31 amino acids at N-terminus and seven Ig-like domains.
胞外区由758个氨基酸组成，包括N端31个氨基酸的信号肽和七个类似免疫球蛋白(Ig)的结构域。收藏指正
4.Results: The correct insertion of PTA1 extracellular region and splicer donor sequence into pIG vector was identified. After transfection of pPTA1/Ig into COS 7 cells, a M r83 000 secreting protein was obtained by anti PTA1 mAb affinity chromatography, and this protein could also be recognized by both anti PTA1 and anti hIgFc mAb.
结果：经序列测定后证实重组表达载体含有正确的PTA1胞膜外区序列及剪接供体序列，转染COS?7细胞后，通过亲和层析纯化证实融合蛋白的Mr为83000，并能被抗PTA1胞膜外区单抗及抗人IgGFc的单抗同时识别。收藏指正
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