3.Methods: High performance liquid chromatography detection (HPLC) was used, using a ODS C18 column (250 mm×4.6 mm, 5 μm) set at 35℃, methanol-water (35∶65) as mobile phase with flowing rate 0.8 ml/min and detected at 210 nm.
4.The separation was performed on an analytical 150mm × 6mm id,Shim - pack CLC - ODS(10μm, particle size) colum. The mobile phase was methanol -water(35:65). The UV detector was set at 365nm.
5.The analytical column(150mm×4. 6mm) was packed with Hypersil BDS C_(18)(5μm) ; The ana-lytical mobile phase was composed of methanol and phosphate buffer(35 : 65,V/V). The determination wave-length was 230 nm.
6.The coating formu-lated has the hardness more than 0.8,impactstrength 4.9N·m,flexibility 1 mm,adhesion100%,UV filterability 80~90%,IR filterabili-ty 35~65% and visible light filterability20~75%.
7.METHODS:A colum of μ Bondapak C 18 (300×4.6mm,10μm) was used with mobile phase of 0.8% triethylamine(pH=3.0) acetonitrile(35∶65) and detected at 215nm,the internal standard was.
8.METHODS The separation was performed on an Agilent XDB-C_8 column(4.6 mm×150 mm,5 μm),and the mobile phase consisted of acetonitrile and 0.1% trifluoroacetic acid solution(adjusted to pH 3.9 with triethylamine)(35∶65)at the flow rate of 1.0 mL·min-1.The detection was carried out at 250 nm.
