• 基本释义

1.4. AFLP markers are exploited in map construction. 140 ploymorphic bands are generated by 17 primer pairs in EcoRI/Msel and Pstl/Msel eynzem combinations.
4．利用EcoRI/MseI和PstI/MseI两种酶切组合，分别筛选了64种引物组合，9对来自EcoRI/MseI酶切组合的引物和8对来自PstI/MseI酶切组合的引物进行了群体分析。收藏指正
2.469 Pstl/Msel primer combinations were analysed in cDNA of male sterile and fertile anthers, as a result, a mean of 18.8 amplification products were detected from the above cDNA templates and 232 differentially expressed transcript derived fragments (TDFs) have been identified.
在所统计的144对引物组合扩增结果中，在gobp至622bp之间的扩增条带数共为2712条，平均每对引物了扩增出18.8条带纹。 在所筛选的469对AFLP引物组合中合在可育株与不育株间表现多态，筛选出232条差异片段。收藏指正
3.Twenty-one AFLP primer combinations of PstlI/Msel were used in the study and 641 AFLP amplified bands were obtained, including 586 polymorphic bands with an average of 30.52 per primer combination. The polymorphism percentage was 91.4%.
结果表明21个PstI／MseI引物组合检出可辨认带共641条，其中具多态性带586条，平均每对引物得到30.52条多态性带，多态性91.4％。收藏指正
4.Selective amplification primer combination patterns EcoRI+2/Msel+3 , EcoRI+3/MseI+3 were both suitable to polymorphism analysis of peach genome. The result showed that the AFLP system was can be used for the genome of peach.
籽预扩增产物稀释20倍用作选择性扩增的模板，选用EcoRI+2/MseI+3、EcoRI+3/MseI+3所组成的128对引物组合进行扩增，扩增条带清晰、多态性高，适合桃基因组分析。收藏指正
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