Motility of spermatozoa
6.So this is the best method of IVF of C57BL/6 and DBA while the oocytes surrounded by cumulus cells of CD-1,B6DF1,FVB are directly used to IVF in the tests of fresh spermatozoa.
7.Acro-some morphology of unfixed, unstained spermatozoa was assessed at100× magnification and fnotility rating was done at 250× with a phase-contrast microscope.

