1.The results of SDS PAGE showed that deletions of the whole 5′flanking region ( argSΔ1 ) or the region in front of ShineDalgarnoSequence ( argSΔ2) or the -10 region of promoter ( argSΔ3 ), caused no overexpression of argS .
2.The distance and the composition between the Shine-Dalgarno(SD)sequence and the start codon have, profound effect on the expression of prochymosin gene causing a 15fold difference.
