Significant growth inhibition was observed in SPC-A1 cell line expressing ASB-8ΔSB on day 4 and persisted to day 6, compared with mock transfected cells ( P <0.01). No difference in the growth properties was observed between ASB-8 and mock transfected cells.
2.ResultsExpression of Fas mRNA and protein in CTLL 2 cells was reduced to 50% after transfection with anti Fas ribozyme. Being treated with anti Fas antibody (JO 2 ), compared with control and mock transfected cells, viability of CTLL 2 cells transfected with anti Fas ribozyme increased by 1 fold, caspase 3 activity and apoptosis rate of ribozyme transfected cells decreased to 50% and 37%, respectively, and cell killing activity was enhanced by 2 fold.
3.MTT assay showed that the IC50 values for cDDP and ADM were re duced to approximate 86.2%and 99.2%in the transfected cells compared with the untransfected cells, respectively. The low concentration of chemotherapeutic dru gs (1.25 ?ol/L cDDP or 0.05 ?ol/L ADM) without inhibition essentially suppre ssed the growth of the transfected cells markedly.
4.Results:PSVL hFASL transfected cells had the expression of human FAS ligand mRNA, PSVL mock transfected cells was negative There is expression of FAS antigen on the surface of U937 cells With U937 cell line as target, the activity of recombinant human FAS ligand (rhFASL) was assayed After coculture of U937 cells with FASL transfected COS 7, the target cells appeared apoptosis phenomenom Conclusion:Recombinant FasL on the surface of COS 7 cells can be used as functional study
5.Results There was a significant reduction of tumor size as compared with that in other six control groups ( P<0 001,P<0 05 ). Between animals treated with liptk ACK and tk ACV,there was a significant difference ( P <0 02)). No significant difference was observed in terms of growth rate of tumors among other six control groups ( P <0 05).
8.3)Construction of nine eucaryotic expressing plasmids carrying the DF1-9,named pCMV/DF1-9. 4) HL60 cells were transfected with pCMV/DFl-9,and the growth inhibition of the transfe-ctant pCMV/DF4-HL60 was observed.
