Southern hybridization
1.PCR, sequencing of the PCR products, PCR-Southern hybridization and Southern hybridization analysis of TO and T1 plants revealed that we had got 8 Gui 99 transgenic plants and 113 TP309 transgenic plants.
2.Genomic DNA Southern hybridization analysis suggest the existence of a single DFL gene in Dendranthema lavandulifolium genome.
3. Results The recombinant plasmids which contained the SSU rDNA fragment were further identified by EcoR Ⅰ digestion,PCR amplification and Southern hybridization.
4.The Study on cloning homogeneous CBS-700(bp) DNA fragment and Southern hybridization of Different Groups from Local Changbaishan Apis Cerana ceranas
5.Southern hybridization showed that OPC08578 had the same distribution in the genome W、H and F. But it has no hybridization signals detected in ABD、R、V、E、St、Ns、I、D and A genomes.
6.The number of recombinant phages is 4.8×105 .Using two synthetic fragments as pro be, positive clone was obtained by in situ hybridization of λ plaques,and a lso identified by restrict enzyme digestion and Southern hybridization.
7.The Bt gene has been successfully conformed to the transgenic triploid of Chinese white poplar and the foreign gene of all the clones were one copy detected by Southern hybridization .
8.Southern hybridization result revealed polymorphisms among their atp A, atp 9, cob and cox Ⅱ genes, while the organizations of mtDNAs from the maintainer line and the restorer line were very similar.
9.PCR electrophoresis assay and PCR-Southern hybridization showed the foreignbar gene had been stably transformed into the genomes of the transgenic wheat plantletsregenerated from 4 of the 9 ppt-resistant calluses.
10.6. The library was screened by the RGA probe of Hvrgak4. Four positive clones were obtained among 1.2 X 106 clones in the library through two rounds southern hybridization.
