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1.① After 21 days, many lipid droplet of high refractivity occurred in many cytoplasm in the treatment group, the Sudan fat stain showed orange, and Kossa method also detected a few osteogenesis, approximately 70% ?28/40? and 10% ?4/40? of the MSCs were transformed into adipocytic and osteogenetic cells in medium containing adipogenesis supplement medium respectively.
①在经过21d成脂诱导培养后实验组的许多细胞胞浆内均出现较多的高折光性的脂滴,苏丹脂肪染色呈橘红色,同时Kossa法也检测出少许成骨分化,其比例为70%(28/40)干细胞可转化为含有脂滴的脂肪细胞; 10%(4/40)干细胞转化为含有钙结节的成骨细胞。收藏指正
2.Ice-sections stained with Sudan Ⅲ that only mesos layer is stained orange indicated that mesos layer contains a lot of lipid and so mesos layer may contribute to the establishment of the permeability barrier.
此外，冰冻切片，苏凡Ⅲ染色，仅中层染为鲜艳橙色，显示中层不但存在，而且是富含脂类物质的结构，因此，中层可能对荒漠沙蜥皮肤防止水分蒸发的渗透屏障的建立起重要作用。收藏指正
3.The concentration limit of Acid orange Ⅱ was 0.04mg/kg,Sudan Ⅰ 0.04 mg/kg,Sudan Ⅱ 0.07 mg/kg,SudanⅢ0.05 mg/kg,Sudan Ⅳ 0.08 mg/kg.
最低检出浓度为:金橙Ⅱ0.04 mg/kg,苏丹红Ⅰ0.04 mg/kg,苏丹红Ⅱ0.07 mg/kg,苏丹红Ⅲ0.05 mg/kg,苏丹红Ⅳ0.08 mg/kg。收藏指正
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