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1.Methods:Drug sensitivity and proliferation of leukemic cells in vitro were determined by leukemic cell colony forming unit (CUF L),MTT drug sensitive test, percentage of S phase cells in cell cycle (S%), fluorescent index(FI) and drug resistant index (DRI) by detecting intracellular daunorubicin, expression of P 170 glycoprotein by APAAP assay, and abundance of bcl XL mRNA by semiquantitative reverse transcription polymerase chain reaction (RT PCR).
材料和方法 :用白血病祖细胞培养(CFU L) ,MTT药物敏感试验 ,细胞周期中S期细胞百分比 (S % ) ,细胞内柔红霉素 (DNR)测定其荧光指数 (FI)和耐药指数 (DRI) ,APAAP法测P 170糖蛋白表达以及半定量RT PCR检测Bcl XLmRNA表达丰度 ,评价AML细胞的药物敏感程度和增殖情况。收藏指正
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