adventitious embryo
1.5 ppm 6-BA, 20 ppm 6-BA, 5 ppm 2,4-D+20 ppm 6-BA, 20ppm2,4-D +20 ppm 6-BA, 10 ppm 2,4-D+20 ppm 6-BA all could lead to the frequency rising and reaching very significant level by spike-spraying. The frequency of adventitious embryos obviously increased to the level of signifcance also by spraying with 5 ppm 6-BA and 5 ppm 2, 4-D+20 ppm 6-BA respectively, which indicated that there were close interrelation between the development of adventitious embryo and the level of growth regulator.
2.STUDIES ON MUTATION BREEDING OF FRUIT TREES IN VITRO——1. Origin and Induction of Adventitious Shoots and Branches in Cultured Embryo of Apple and Pear
3.(2) The best proliferation medium of adventitious shoots was WPM+BA 0.3 -0.5mg/L+ sucrose 20g/L + agar 6g/L. Seven to twenty shoots were able to obtain from one embryo in the first subculture.
