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1.In this paper,we develop the experimental process by substituting 96-microplate for erlenmeyer flask with one notch to complete the assay.
采用 96孔培养板替代三角锥瓶对酵母筛选法进行了改进 .收藏指正
2.By the assay of Bradford and Glyko BandScan, we detected that the yield of hBMP4 expressed in shaken flask was 33. 34 mg/L and amounted to 50. 4% of the total protein in the supernatant. The yield of hBMP7 was 13. 76 mg/L and amounted to 20. 8% of the total protein in the supernatant.
利用Bradford蛋白质定量法并结合Glyko BandScan量化软件的分析发现，表达上清中hBMP4表达量达到33.34mg／L，占总蛋白含量的50.4％，hBMP7的表达量达到13.76mg／L，占总蛋白含量的20.8％。收藏指正
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