assay preparation
2.Using the membrane prolactin receptor preparation from rat liver as specific binding protein,we developed a sensitive radioreceptor assay(RRA)for human prolactin.
3.coli RRI. After heat induction,a high level expression of fusion protein was achieved and the recombinant protein MS2 mIL 5 consists about 40% of the total soluble protein as detected by SDS PAGE assay. The fusion protein was purified to about 90% purity by extracting the inclusion bodies with Triton X 100 and urea,and has been used for preparation of anti mIL 5 monoclonal antibody.
4.During the artifical antigen preparation, room temperature oximation reaction monitored by thin layer chromatography (TLC) assay was used to get AFB_1-Oximation (AFB_1-O) instead of heating flux oximation reaction, the yield was up to 95%.
5.It can be concluded that digestion of endometrium with 1.25 g/L trypsin for 8～9 h (0～4℃) was the optimal condition for preparation of EML from pregnant rabbit and the activation of EML obtained could be conveniently tested with MTT assay after 48 hours of culture in vitro.
6.From the information available for the preparation to be assayed (the “Unknown”),assign to it an assumed potency per unit weight or volume,and on this assumption prepare on the day of the assay a stock solution and test dilution as specified for each antibiotic but with the same final diluent as used for the USP Reference Standard.The assay with 5levels of the Standard requires only one level of the Unknown at a concentration assumed equal to the median level of the Standard.
