average assay
3.ResultsThe chomosone number of NFS 60 cell was 39, about 1×10 5 cells/ml to 1×10 7 cells/ml,the dying color showed gradient when the NFS 60 cells was dyed by MTT,the method(4,4) was adopted in the determination of rhG CSF biological activity,the average FL% of potency was 13.560% for single expriment,and the CV of inter assay and intra assay was lower than 10% and 10.109%,respectively.
6.We used butylphenyl formate was selected as an extemal standard. Result The average recoveries of methoxamine phosphate,DDVP and Rogor were (96.3±4 8)% and (100 0±3 3)% and (98 2±2 8)%,respectively, The minimal limits of assay were 0.01μg,0 02μg and 0 02μg for methoxamine phosphate, DDVP and Roger.
9.ObjectiveTo establish a New Vierordt mothod of the assay of individual medicaments in the chloramphenicol and salicylic acid t incture Method The new Vierordt method need not separation , and detect directly at 278 nm, 298 nm respectively Result The average recoveries were 99 6%,100 6%, with RSD 0 87%,1 13% respectiv ely Conclusion This determination method is simple,rapid,accura te and suitable for hospital pharmacy
10.The species of bacteria were evaluated by routine method and the endotoxin was detected by gel-colt assay with limulus reagents. Results:The average bacteria colony forming unit per milliliter in reverse osmosis and dialysis fluid from the dialyzer inlet,outlet,fluid A,and fluid B was 130 CFU/ml,259 CFU/ml,644 CFU/ml,13 CFU/ml,and 268 CFU/ml,respectively. The positive rate of endotoxin was 5.8 in reverse osmosis.
