4.Methods :Clinical bloodtumor samples were se parated to obtain mononuclear cells by Ficoll-Hypaque,solid tumor tissues were made to be single c ells by using mechanical method.
5.Methods Using the RT-PCR method, the SSX-1 and NY-ESO-lmRNA were examined in the peripheral blood, tumor tissues, para-tumor tissues of 25 cases of HCC patients and samples corresponding with them of 20 control-patients.
7.Objective:To analyse the efficiency and influence factors of collecting mononuclear cells(MNC) from the peripheral blood of tumor patients with COBE Spectra AutoPBSC(Version 6.1) system.
8.Change of tumo r volume and tumor growth inhibiting rate,blood perfusion of tumor,expression of VEGF mRNA in tumor tissue,serum VEGF level,SSTR1-5 mRNA expression and tumor c ells apoptosis were evaluated.
9.As it is all known that,growth and metastasis of tumor depend on the forming of newborn blood vessel, and tumor cells can secrete vascular endothelial growth factor(VEGF) and its receptor which accelerate the forming of newborn blood vessel.
