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1.In ligand combination experiment, we found quantitis of expression of membrane-bound protein c-mpl in 100 μg/L rhIL-13 group increased by 28.5% ( P<0.05 ).
经 10 0 μg/LrhIL - 13处理过的实验组Dami细胞膜蛋白c -mpl的表达比对照组高 2 8 5 % (P <0 0 5 )。收藏指正
2.The results of Western blotting showed that the 120 and 170kDa proteins were the main ones that bound Cry 1 Ac in the BBMV, and the protein at 120kDa was the APN anchored by GPL By using ligand blotting test, a 120kDa protein in BBMV was recognized binding with 5I-Cryl Ac, it was reconfirmed that the 120kDa APN is the receptor for Cry 1 Ac.
Western杂交证明棉铃虫中肠BBMV上结合Cry1Ac的主要是120和170kDa的蛋白,并且120 kDa蛋白是通过GPI锚定的APN。 通过配体杂交试验,得到~(125)I-Cry1Ac与BBMV杂交的120kDa条带,再次确认这个120kDa的APN是Cry1Ac的受体蛋白。收藏指正
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