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1.C3d-P28 coding gene was amplified by PCR.
将PCR获得的补体C3d-P28 DNA序列以头尾串连的方式构建四聚体。收藏指正
2.Expression and Purification of the Fusion Proteins hCGβ-(CTP)_4 and hCGβ-(CTP)_4-(C3d-P28)_4 in E.coli
hCGβ-(CTP)_4和hCGβ-(CTP)_4-(C3d-P28)_4在大肠杆菌中的表达及纯化收藏指正
3.Objective:To investigate the prokaryotic expression of the genes encoding proteins hCGβ- (CTP)_4 and hCGβ-(CTP)_4-(C3d-P28)_4 and to purify the recombinant proteins.
目的:在大肠杆菌BL21(DE3)中表达hCGβ-(CTP)_4、hCGβ-(CTP)_4-(C3d-P28)_4融合蛋白,并对蛋白进行分离纯化。收藏指正
4.Conclusion:The recombinant proteins were successfully expressed in BL21(DE3)and purified. This study provides necessary data for further investigation of the immunogenicity and anti-cancer vaccine of fusion protein hCGβ-(CTP)_4-(C3d-P28)_4.
结论:在BL21 (DE3)中成功表达了hCGβ-(CTP)_4和hCGβ-(CTP)_4-(C3d-P28)_4融合蛋白,并确立了纯化方法,为进一步研究其免疫原性和应用于抗肿瘤疫苗奠定了基础。收藏指正
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