1.But calluses are another matter.
可足茧问题就没那么容易克服了。收藏指正
2.form a callus or calluses.
形成茧或皮肤的硬结组织。收藏指正
3."It was really interesting to me to lose the calluses from the bottom of your feet and to get calluses on the top of your feet after being up here for a few months," she said.
佩吉说:"在这里呆上几个月,脚底的老茧都不见了,他们全部都长到了脚背上,这对我来说实在是有趣之极。收藏指正
4.Alfalfa calluses were induced with diethylsulfate (DES). The DES concentration leading to 50% death was confirmed, and cold-resistant mutants were screened out at -7℃.
对诱导的苜蓿Medicagosativa愈伤组织进行不同浓度的硫酸二乙酯 (DES)诱变处理 ,确定其半致死浓度 ,在 - 7℃低温下进行筛选 ,以获得抗寒性突变体 ;收藏指正
5.The result showed that the mutant calluses could retain the relative growth rate at 14.5%,12.8% and 41.8%,when they were planted on the media contain 20% mannitol,1.5% NaCl or 20% PEG-6000,while the control calluses could not grow under each of these stress circumstances.
突变细胞系可以在对照不能生长的20%甘露醇、1.5%NaCl和20%PEG-6000胁迫条件下,分别表现出14.5%,12.8%,41.8%的相对生长量;收藏指正
6.[Results] (1) After the three kinds of basic medium were added with the phytohormones of 0.1mg/L kinetin (KT) and 0.2mg/L α-naphthalene acetic acid (NAA) , a large amount of ACB calluses were induced in the MS medium and modified MS medium, while ACB calluses did not occur in the 1/2 MS medium.
【结果】①在3种基本培养基中加入相同的植物激素(0.1mg/L的KT和0.2mg/L的NAA)后,MS和改良MS培养基均能诱导大量的愈伤组织,而1/2MS培养基则未能诱导;收藏指正
7.PCR electrophoresis assay and PCR-Southern hybridization showed the foreignbar gene had been stably transformed into the genomes of the transgenic wheat plantletsregenerated from 4 of the 9 ppt-resistant calluses.
PCR电泳检测与PCR-Southern杂交结果显示,外源bar基因已转化进了由其中4块愈伤组织再生出的转基因的小麦植株中。收藏指正
8.After transferring to MS medium supplemented with 1 mg/L ABA, 50 mg/L Kan and 100 mg/L Carb, these embryogenic calluses formed 13 plantlets via somatic embry ogenesis.
将这些抗Kan胚性愈伤组织转移到添加1mg/LABA、50mg/LKan和100mg/LCarb的MS固体培养基上,通过体细胞胚胎发生途径获得了13株再生植株。收藏指正
9.The optimal medium for adventitious bud differentiation from leaf-derived calluses was found to be MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+GA3 3.0 mg/L for " DE02-24-24" ;
JC02-27-2的叶片愈伤组织诱导的最佳培养基为MS+6-BA 3.0 mg/L+NAA 0.15 mg/L。 愈伤组织的诱导率均可达到100%。收藏指正
10.the optimal medium for inducing calluses from the stems of Chinese date was MS+1.0 mg·L~(-1) 2,4-D + 0.5 mg·L~(-1) 6-BA;
茎段为M S+2,4-D 1.0 m g. L-1+6-BA 0.5 m g.收藏指正
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