capacitation of spermatozoa
1.So this is the best method of IVF of C57BL/6 and DBA while the oocytes surrounded by cumulus cells of CD-1,B6DF1,FVB are directly used to IVF in the tests of fresh spermatozoa.
2.Acro-some morphology of unfixed, unstained spermatozoa was assessed at100× magnification and fnotility rating was done at 250× with a phase-contrast microscope.
3.The fertilization rates of the same strains oocytes inseminated with frozen-thawed spermatozoa were 68.4%, 33.7%, 47.2%, 27.8% and 9.0% respectively in KM, ICR, DBA/2J, BALB/c and C57BL/6J mouse.
4.The high specific activity (1.9 uCi//ug) 125I-TGL was used to bind human spermatozoa. The binding of TGL to its receptors is a saturation process with respect to the protein, and the scathard plot is nonlinear that suggested the conformation change of membrane components because of the presence of coopera-tivity.
5.The [Ca~(2+)]_i in sperm was decreased after the spermatozoa were treated with the inhibitors of adenylyl cyclase, phospholipase C or protein tyrosine kinase compared with the sperm treated with E_2-BSA only.
6.The 60.61% FBI exclusion and 72.73% maturation ratio had been obtained with the above cultured conditions. We had adopted BO and TALP medium with heparin as sperm in vitro capacitation andfertilization medium.
