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1.cark and care
烦恼与忧虑收藏指正
2.In the cark he ran up against a stool.
他在黑暗中撞上丁一只凳子。收藏指正
3.Methods Primary neonatal cardiomyocytes were transiently transfected with 2×NFκB Luciferase or 2 6kb Nppa promoter Luciferase reporter genes with or without CARK expression vectors. After transfection, the cells were treated with or without Ang Ⅱ for 24hours.
方法 :构建CARK真核表达载体 ,用脂质体介导的方法将CARK基因与NFκB Luciferase报告载体或 2 6kbNppapromoter luciferase报告载体共转染入原代培养的心肌细胞中 ,检测荧光素酶活性的变化。收藏指正
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