cauliflower mosaic virus
1.COMPLETE NUCLEOTIDE SEQUENCE OF CAULIFLOWER MOSAIC VIRUS ( XINJING ISOLATE ) GENOMIC DNA
2.A1200 and pRT! 05. The Suuccki cDNA library were driven by the cauliflower mosaic virus (CaMV) 35S promoter.
3.A full-length cDNA of DFL and its antisense nucleotide sequence have been transformed into Nicotiana tabacum under the control of a cauliflower mosaic virus 35S promoter.
4.The multiplex PCR detection methods of endogenous PAPA gene, Cauliflower mosaic virus (CaMV) 35S promoter, nos terminator and nptⅡ gene were developed, using the mixture of potato DNA and positive plamid pBI121 as PCR template.
5.The genetically engineered cross protection which was supplied by weak strain Bari 1 gene VI of cauliflower mosaic virus(CaMV),and its genetic regularity were studied with Brassica crops( Brassica campestris ssp. rapifera,B.campestris ssp. pekinensis,B.campestris var. parachinensis,B.campestris var. purpurea,B.juncea,B.oleracea var. botrytic )which were transformed with CaMV gene VI.
6.The plant expression vector pBIN19-ROK219 was constructed, which contains cauliflower mosaic virus (CaMV) 35S promoter and noster. Introduction of IBDV VP2 gene under control of CaMV 35S promoter resulted in the construction of binary expression vector pBR-VP2. Then the VP2 gene was in the left and right border regions, which denote the limits of the DNA that is integrated into the plant genomic DNA via Agrobacterium fwme/ac/ms-mediated transformation.
7.Agrobacterium tumefaciens strain GV3101 mediated transgenic plants containing CABB BJI gene Ⅵ and Bari 1 gene Ⅵ were obtained in Arabidopsis thaliana by using the method of vaccum infiltration through the cloning and transformation of gene Ⅵ of cauliflower mosaic virus (CaMV) strains CABB BJI and Bari 1.The molecular assay showed that the gene Ⅵ was integrated into plant genomes of Arabidopsis thaliana and had expressions of various degrees.
8.Transgenic tobacco plants expressing a stress responsive gene BoRS1, isolated from Brassica oleracea var. acephala, under the control of the 35S promoter of the Cauliflower mosaic virus were produced. Some plants were further used to test the effect of high level BoRS1 expression on drought stress resistance. The presence of transgene in putative transgenic plants was confirmed by PCR analysis.
9.588 samples of Chinese cabbage (Brassis campestris), non-heading Chinese cabbage ( B. chinensis), radish (Raphanus sativus), cabbage (B.oleracea) and cauliflower (B. o. var. botrytis) were collected from Beijing area, 69.21% of the samples contained Turnip Mosaic Virus (TuMV) .
