cell hybridization
1.Methods:We investigated chromosomal DNA copy number changes in 3 ESCC cell lines by comparative genomic hybridization(CGH).
2.The results of localization of mRNA experessing cell by in situ hybridization demonstrat- ed the significant increase of mRNA of ET-1,ETA, ETB expression in all organs, The adhesion force between a single PMN and HUVEC pair increased if the EC or PMN incubated with burn sera, and it was reduced when CD11a/CD18mAbandCD11a/CD18mAbwere usd.
3.Hybridized cell lines ( CNf-A/B95-8 ) were obtained by hybridization of CNf-A cell with B95-8 cell and EBNA was detected in these hybridized cell lines.
4.Methods 3H TdR incorporation and cell counting for the assay of cell growth; dot blot, in situ hybridization, and immunohistochemical methods for analysing the expression of cyclin D1 and cycle dependent kinase 4 (CDK4) genes.
5.(2) Implanted cell fates were traced by Y chromosome fluorescence in situ hybridization and some transduced gene cells, direct involvement in bone formation was observed;
6.Comparative Genomic Hybridization Analysis of Nasopharyngeal Carcinoma Drug-Resistant Cell CNE2/DDP and Its Parent Cell CNE2
7.Methods: The expressions of hTR and hTERT in the lesions of patients with psoriasis, basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and seborrheic keratosis (SK) were examined with in situ hybridization. Using immunohistochemistry methods sequential sections were stained with anti-Ki-67 antibody, a proliferative marker.
8.Methods The hepatocellular carcinoma cell lines HCC 9903, EGHC 9901,HCC 9901 and HCC 9810 were respectively detected by fluorescence in situ hybridization (FISH) centromere DNA probes of X chromosome.
9.Neuroethology were evaluated with BWT scoring standard,and regional cerebral blood flow (rCBF)were measured,the expresses of GAP-43,SYN in the brain tissues of experimental rats and the change of ET-1? ecNOS? P-gp mRNA in the brain microvessel endothelial cell of experimental rats were observed by means of immunohistochemical method and hybridization in situ.
10.Methods: IFN-γ expression was determined by in situ hybridization in 48 cutaneous squamous cell carcinomas (SCC), 41 basal cell carcinomas (BCC), 15 keratoacanthomas (KA) and 10 seborrheic keratoses (SK).
