challenge dose
2.Results showed that F1 antibody in serum peaked rapidly af-ter fundamental immunity 20 dyas with GMT of 114,and the bacterin induced enough immunogenicity for all the guinea pigs against the challenge exposure with 1 billion cells of the virulent strain 141 with a protective rate of 100%. The challenge dose,with better immunizing effect,was 105 times more than that in the immuni-ty test of biological product rules.
4.Methods: One LD 50 dose of LPS was injected to challenge mice, and different dose of BPI modeling peptides was injected within 20 s after the challenging and then 5 mg·kg -1 /10 mg·kg -1 dose of BPI modeling peptides was injected pre/post the challenging respectively, to observe the protective or preventive effects of BPI modeling peptides on the LPS challenged mice.

