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1.The method was compared with ABC method and dextran coated charcoal bio-chemical assay (DCC).
以抗雌二醇ABC免疫组化法(ABC法)和葡聚糖包裹活性生化法(DCC法)作为E_2-HRP法的对照。收藏指正
2.Cytosol and nuclear estrogen (ERc, ERn) and progesterone (PRc, PRn) receptors were measured with the Dextran-coated Charcoal method in 50 ovarian tumors and 6 normal ovarian specimens.
并用葡聚糖─活性碳吸附法(DCC)测定了50例卵巢肿瘤和6例正常卵巢组织胞浆雌(ERc)、孕(PRc)激素受体和胞核雌(ERn)、孕(PRn)激素受体。收藏指正
3.Methods:ER and PR expression status in endometrial carcinoma in 68 cases were measured using Dextran Coated Charcoal (DCC). Among 30 of these cases, immunohistochemistry method was also used to measure the ER and PR expression status.
方法:采用葡聚糖-活性吸附法(DCC法)对68例子宫内膜癌组织进行ER、PR测定,同时采用免疫组化法对其中30例进行ER、PR检测。收藏指正
4.The correlation of serum estradiol concentration and uterine estrogen receptor (ER) gene expression (ERn and ERc quantitated by Dextran Coat Charcoal assay and mRNA by Northern blotting) was studied during the rat estrous cycle and early pregnant stage (d1~d10).
本文对大鼠在正常动情期和早期妊娠阶段(d1~d10)外周血雌二醇(E2)水平和子宫中雌二醇受体(ER)基因的表达(以3H-E2配体结合饱和分析测定胞浆受体ERc和核受体ERn,以Northern印迹法测定ERmRNA)作了全面的分析。收藏指正
5.1. Proliferation assay of MCF-7 human breast cancer cells: cells(2-3 x104 cells/ml) were seeded in culture plates of 96 wells. Each well contained 100ul RPMI-1640 medium(phenol red-free) supplemented with 10% dextran/Charcoal-treated fetal bovine serum (FBS) and insulin(30u/mL).
1.MCF一7人乳腺癌细胞增殖实验:用无酚红的RPMI一1640培养基(内含活性一葡聚糖处理的10%胎牛血清,30U/100ml胰岛素)调整细胞浓度为20000一30000/ml,接种于96孔培养板,每孔接种100 pl。收藏指正
6.[WT5”HZ][ST5”HZ]Methods:[WT5”BZ][ST5”BZ]Dextran Coated Charcoal(DCC)method was performed to quantitively detect the level of ER. Twenty five female rats of 4 week old were selected and were sacrificed after 3 days,1 week,2 week,3 week and 4 week.
材料和方法 :选用 2 5只 4周龄、雌性SD大鼠 ,随机分成 5组 ,分别于 3天、1周、2周、3周、4周后将动物处死 ,应用葡聚糖—活性吸附法 (DCC法 )进行髁突软骨的雌激素受体的测定。收藏指正
7.Estrogen receptors (ER) of the spayed rat uterus were measured by modified dextran coated charcoal assay(DCC) ,after injection of estradiol (E) or simultaneous injection of estradiol and tamoxifen (E+T) 6h to 64 days respectively. The level of ER in E group and E+T group were both increased with the extention of the time after the injection.
利用改进的葡聚糖活性饱和分析法(DCC法),对卵巢切除大鼠注射雌二醇(E组)或同时注射雌二醇和三苯氧胺(E+T组)后6h至64d,进行子宫ER含量测定,发现,E+T组比E组的ER值小并且增加缓慢;收藏指正
8.In 56 cases the PAP method was compared with the ABO (avidin-biotin-peroxidase complex) immuno-histoehemical method and DGO (dextran coated charcoal) biochemical assay in the same specimens. Comparison of the PAP and DCC methods showed a total correspondence rate of 71.4% and a correspondence rate of 86.8% in positive cases.
卵白素、生物素、过氧化物酶标记法(ABc)和葡聚糖衣活性法(DCC)三种方法作比较,发现PAP与DCC法的总符合率为71.4%,阳性符合率为86.6%。收藏指正
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