chelex-100
1.Conclusion: Chelex-100 assay was simple and conventional in extraction of bacteria DNA.
2.2.Chelex-100 and Chris-Hcl were adopted to extract DNA from buccal mucosa swabs. followed by purifying the PCR products.
3.The result of secondary-level speciation were obtained by using cation-exchange resin, Amberlite XAD-2 macroreticular resin and Chelex-100 chelating resin. Four elements almost existed on free ions with little non free ions.
4.Here,A TaqMan technology of Real-time PCR and a conventional PCR were developed to detect the bovine material in the MBM. Template DNA of bovine was extracted from MBM using Chelex-100,and this step spent only about 20 minutes. The conventional PCR could detect the 0.001?
5.To explore a simple and easy method to detect the minimal residual disease (MRD) in children with acute lymphoblastic leukemia (ALL), DNA was extracted from stored bone marrow smears with chelex 100 at different periods in 41 children with ALL. Using TCR Vδ 2 Dδ 3 rearrangement fragments as gene markers, and PCR amplification of quantitative method of limited dilution, the relationship between MRD levels and clinical progress was dynamically monitored.
