chimera mouse
1.The results showed that the engineered chimera polypeptide exerted obvious inhibitory activity to α amylase from digestive tube of the roach ( Periplaneta americana ) at pH 5.5 with the concentration of 9.5×10 -5 mol/L, and also exerted 36% of the neurotoxic activity of the natural huwentoxin I as shown by the experiments of blockage of the neuromuscular transmission of isolated mouse phrenic nerve diaphragm preparations.
2.After treated with recombinant mouse RAGE/Fc chimera (rmRAGE/Fc)/HMGB1, anti-RAGE antibody/HMGB1, HMGB1 or nonimmune rabbit IgG, the phagocytosis was determined by the neutral red dye uptake, the cytotoxicity to L1210 cells was measured with MTT assay, the chemotaxis was determined by use of Costar Transwell cell culture chamber inserts, and the expression of I-Ak MHC classⅡantigen on macrophages was detected by flow cytometry.
