chromatographic assay
1.FAST SEPARATION OF ESCHERICHIA COLI O_ (157 ) ∶H_7 WITH DOT IMMUNO CHROMATOGRAPHIC ASSAY AND IMMUNIZE MAGNETIC SAND.
2.Method: Gallocatechin, catechin and epicatechin are used as reference substances. Chromatographic assay is performed on a C_(18) colum. The mobile phase is composed of methanol and acetic acid solution with gradient elution.
3.Objective:To elucidate the imcompatible mechanism of traditional Chinese Medicine,the effect of Aconitum coadministration with Lilac Daphne on the main enzyme activity,such as CYP1A2,CYP2E1 and CYP3A1/2.Methods:CYP1A2 and CYP2E1 activities were quantitated by high performance liquid chromatographic(HPLC) assay;
4.This includes the extraction and clea n-up procedures and chromatographic methods (HPLL,GC) applied and the immunoche mical method,enzyme-linked immunosorbent assay (ELISA),employed for the determi nation of these mycotoxins.
5.Methods TLC was used to identify Golden cypress,Gardenia,Curcuma,Caltrop in Xieriga Siweitang capsules,HPLC was used to assay the content of hydrochloric berberine in Xieriga Siweitang capsules,the chromatographic column was Kromatosil C18,the mobile phase was acetonitrile:0.03mol/Lsodium dihydrogenphosphate:phosphoric acid(35:65:0.08) and the detective wavelength was 265 nm,and the flow rate was 1.0 mL/min.
