chromosome satellite
1.Methods Human specific Y chromosome satellite DNA CEPY and 17 chromosome satellite DNA p17H8 were used as probes for IFISH. The peripheral blood samples from 2 goats transplanted with human male hematopoietic stem cells (HSC), 1 normal negative goat and 1 normal man were analyzed.
2.Objective To find an effective method which can be used for studying variation of X chromosome centromeric alpha satellite DNA.
3.In the S-chromosomes present in TAI7045, there were one satellited chromosome pair, in which a large satellite was present on the short arm and the hybridization signal of S genome was detected over the satellite. The molecular karyotype of TAI7045 revealed by GISH could be depicted 2n = 56 = 42 wheat + 6 S + 2 S-sat + 4 Es + 2 EVS.
5.Relation between Variation of Centromeric Alpha Satellite DNA on Human X Chromosome and Aneuploidy
6.Methods: Biotin labeled alpha satellite X chromosome DNA(pBamX7) probe and Digoxigenin labeled Y chromosome long arm terminal repetitive sequence (pY3. 4) probe in situ hybridized with pre-treated slides of peripheral blood chromosome and in-terphase nucleus.
7.Contrasting the normal karyotype of the diploid Chinese cabbage-pe-tsai,the 25S rDNA hybridization signals were located near the end of 1L,the central section of 2L,the centromere region of 3L and 4L,and the satellite of chromosome 10,respectively.
