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1.Methods:cDNAs encoding two pieces of HER-2/neu receptor ligand domain (RLD) were amplified by PCR, and fused by using the same cleavage site KpnⅠ. The ligated product was inserted into prokaryotic expression vector, and expressed as the fusion protein in E.
方法 :采用PCR技术将HER_2 neuRLD的两段基因分别扩增 ,并利用基因片段末端的共同酶切位点将两段基因相连接 ,连接产物插入原核表达载体 ,在大肠杆菌中实现融合蛋白的表达。收藏指正
2.Purified HMGB1 was eluted from GSTrap FF affinity chromatography after thrombin cleavage. To identified the function of purified proteins, the product was co-cultured with THP1 cells.
经用GSTrapFF蛋白纯化柱和凝血酶行柱上酶切 ,得到纯化的重组蛋白HMGB1,用培养的人单核细胞系THP1检测蛋白活性。收藏指正
3.MPP-a was oxidized with OsO 4 in THF containing catalytic pyridine at 0 ℃ and followed by glycol cleavage with sodium periodate in aqueous THF to give the methyl pyropheophorbide-d (MPP-d) which was reacted with carbon tetrabromide and triphenylphosphine to generate gem-dibromine substituted product at 3 b-position.
用四氧化锇和高碘酸钠将焦脱镁叶绿酸甲酯的 3 位乙烯基氧化成醛 ,进而与四溴化碳和三苯基磷反应 ,生成 3 位偕二溴取代焦脱镁叶绿酸衍生物 .收藏指正
4.Experimental results showed that obvious interaction between Cu(phen)_2 2+ complex and 6-MP occurs. Their binding product has an enhanced interaction with calf thymus DNA compared with that in the absence of 6-MP due to partly intercalative effect. At the same time, 6-MP and Cu(phen)_2 2+ enhanced the pBR322 DNA cleavage by H_2O_2 and ascorbic acid.
结果表明,Cu(phen)22+与6MP发生了明显的相互作用,其作用产物不仅与小牛胸腺DNA具有更强的相互作用,并且在H2O2和抗坏血酸存在下对质粒pBR322DNA具有更强的断裂能力,与DNA的作用模式可能为部分插入模式。收藏指正
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