column fractionation
1.In order to determine the influence of environmental variations on phosphorus removal mechanisms in a combined pond-wetland system, the dominant removal mechanisms of phosphorus in different treatment unit and their influence on phosphate transformation between sediment and water column were investigated through analyzing the differences of phosphorus fractionation with water conditions.
2.Method:The trypsin inhibitor in peanut seeds(PTI)was purified by 50 mmol/L acetic acid,50-90%(V/V) acetone fractionation,followed by passing through DEAE Sephadex A50 ion exchange column.
3.The arylamidase (E. 0. 3.4.11.2. AAD) of mouse kidney was purified by using Triton X-100 and trypsin treatment to solubilize the enzyme, and further combined with the use of ammonium sulfate fractionation and several types of column chroma-tography.
4.THS-1 produced high yield chitinases with high activity in the liquid culture on a rotary shaker induced by colloidal chitin. The chitinases were purified from fermentation filtrate by fractionation with ammonium sulphate in 90% saturation and chromatography on Source 15Q PE 4.6/100 Column and were quantitatively measured by the DNS method.
