competent cell
3.Methods The replication-competent HBV recombinant plasmid pHBV4.1 plus different liver-enriched transcription factor (HNF1,HNF3,HNF4,HNF6,C/EBP and RXRα/PPARα) expression plasmids were co-transfected into nonhepatic cell lines (NIH3T3,HeLa,293T,SW1353,CV-1 and COS1).
4.To construct the Baculovirus expression vector for translationally controlled tumor protein (TCTP) gene of Schistosoma japonicum and to express in insect sf9 cell line, the TCTP eDNA was cloned into plasmid pFASTA, and therecombinant plasmid was transformed to competent cells DH10Bat.A transformant containing the target gene was obtained and named as BacmidGFP-SjTCTP.
