count cycle
3.Method:AFP was collected and purified from human cord serum and incubated with NIH3T3cells and human hepatoma Bel7402cell which cultured in vitro. The methods of MTT count,the anal-ysis of cell cycle phase and 3 H-thymidine deoxyribonucleotide( 3 H-TdR)incorporation were used for check the conditions of cell proliferation.

