1.The white haze of boundary layer was collected. F12-DMEM nutritive medium was used to suspense cells, then the cells were transferred into cultureflask according to the density of 1×109 L-1 with the same culture condition to adherence separation.
3.CONCLUSION: MSCs can be cultivated successfully in vitro with high successful rate and purity when 1×1011 L-1 mononuclear cells were seeded in low-sugar DMEM culture medium containing 5% FBS and a cultureflask coated with FBS.
4.MethodsTo digest the passage 2 and 7 DPCs in log growth phase and to adjust the cell concentration to 3×105 cells/mL and incubate the DPCs to 24 hole cell cultureflask.
5.The control system drives the robot move to the default position where scions have been prepared to plant, and the end-effector grip and transplant the scions into a cultureflask.
10.Cooked rice dumped into the triangular flask, sterilization for 20min under 0.1MPa, then inoculate and 8～9 days culture at 32～35℃ for triangular flaskculture;
