8.The results showed that optimal solid culture medium was that the optimal C source was wheat straw∶wheat surface=4∶6,stuff∶water=1∶2.5,adding 1%(NH4)2SO4,0.1% Tween80,the optimal original pH is 5.0(for FPA) or 3.0(for CMC). The optimal culture temperature was 28 ℃,incubation time was 96 hours.
结果表明:最佳
碳源为小麦秸粉∶麦麸(4∶6),料∶水=1∶2.5,加入1%(NH4)2SO4,0.1%Tween80,初始pH值为5.0(滤
纸酶活)或为3.0(CMC酶活),28℃下培养96 h时其产纤维素酶活力最高.
9.By primary research,the results in shaking flask culture showed that the fermentation broth was loaded in 250 mL Erlenmeyer flasks containing 50 mL of synthetic medium consisting of CMC-Na 0.5 %,peptone 1 %,bran 3 % and incubated at 28 ℃ for 48 h. Then the maximum yield of FPase was 111.5 U/mL.
试验结果表明,以0.5%羧甲基纤维素钠(CMC-Na)为
碳源,1%蛋白胨为氮源,3%麸皮为添加物,在28℃,20%装液量,摇床振荡培养24h后,其发酵液滤
纸酶活(FPA)可达111.5U/mL。