1.Acro-some morphology of unfixed, unstained spermatozoa was assessed at100× magnification and fnotility rating was done at 250× with a phase-contrast microscope.
用相差显微镜不固定、不染色1000倍检查顶体形态,250倍估价活力。
2.The [Ca~(2+)]_i in sperm was decreased after the spermatozoa were treated with the inhibitors of adenylyl cyclase, phospholipase C or protein tyrosine kinase compared with the sperm treated with E_2-BSA only.
应用腺苷酸环化酶、磷脂酶C和酪氨酸蛋白激酶特异性抑制剂分别作用后均明显抑制E_2-BSA引起的胞内Ca~(2+)浓度的上升(P＜0．05)。
3.So this is the best method of IVF of C57BL/6 and DBA while the oocytes surrounded by cumulus cells of CD-1,B6DF1,FVB are directly used to IVF in the tests of fresh spermatozoa.
因此，C57BL／6和DBA小鼠理想的鲜精体外受精方法为切割透明带法，而CD-1、B6DF1、FVB小鼠切割透明带卵的受精率与卵丘卵母细胞复合体的受精率均差异不显著，故此三种品系小鼠则直接用卵丘卵母细胞复合体进行鲜精体外受精法简单有效。
4.The fertilization rates of the same strains oocytes inseminated with frozen-thawed spermatozoa were 68.4%, 33.7%, 47.2%, 27.8% and 9.0% respectively in KM, ICR, DBA/2J, BALB/c and C57BL/6J mouse.
冻融小鼠精于体外受精结果随遗传背景的不同差异显著，受精率分别为：KM小鼠68.4％、ICR小鼠30.3％、DBA／2J小鼠47.2％、C57BL／6J小鼠6.8％、BALB／cA小鼠25％。
5.The high specific activity (1.9 uCi//ug) 125I-TGL was used to bind human spermatozoa. The binding of TGL to its receptors is a saturation process with respect to the protein, and the scathard plot is nonlinear that suggested the conformation change of membrane components because of the presence of coopera-tivity.
用氯胺T法标记~(125)I—白附子凝集素,其比放射性为1 .9微居里/微克,与人精伯胞膜受体的结合呈S型饱和曲线,Scatchard plot非线性,提示受体之间可能存在协同效应。
