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1.Add chemicals to tell the egg to start developing into an embryo. Incubate embryo in a test tube for a few days, then implant it into a foster mother.
将胚胎放入试管中孵化几日，然后将其植入“养母”体内。收藏指正
2.The inhibitory rate of specific egg yolk IgY on adhesion of S.mutans onto surface of glass test tube were 30.03% and 84.89%,respectively,at concetration of 67 μg/ml and 670 μg/ml. It has significant difference when compared to control IgY.
当特异性卵黄IgY抗体浓度为67μg/ml和670μg/ml时,对变形链球菌在玻璃表面黏附的抑制率分别为30.03%和84.89%,与对照IgY抗体比较,有极显著性差异(P<0.001)。收藏指正
3.3. The infective-stage eggs were transferred into an Erlenmeyer flask containing glass beads (diameter 2 mm) withsmooth surface and the egg suspension was stirred at 60 rotations/min for 2-3 min to remove shells. Larvae suspension were transferred onto the surface of Ficoll-Hypaque in a tube, and centrifuged at 1000rpm for 10 min.
3. 将感染期卵洗涤数次后移入含有小玻璃珠（直径2mm）的锥形瓶内，人工振荡2-3分钟，幼虫全部破壳而出，然后将此悬液沿管壁轻轻移至装有淋巴细胞分离液的试管中，悬液加至淋巴细胞分离液的表面，1200rpm 离心10分钟，沉淀层即为幼虫层。收藏指正
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