electron stain
1.Methods Observing the cell structure changes by hematoxylin-eosin stain(HE stain),the cell ultra microstructure changes by electron microscope(EM),neuronal apoptosis by fluorescence microscope; determining the concentration of malondialdehyde(MDA),estrogenic hormone(E_2) and superoxide dismutase(SOD) with biochemistry method or radioimmunoassay(RIA).
4.Environmental samples were cultured in a special selective enrichment culture for nitrite-oxidizing bacteria, and identified by biochemical methods, gram-stain reaction, PCR and electron microscope. 10 strains of bacteria were screened and isolated by enrichment culture from soil and sewage, and were named X01～X10 in turn, which were all gram-negative microorganisms and rod or cocci bacteria.
