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1.The Background was done the same way as the skin, no manual selecting, only magic marker tool and faded down to 50% opacity at the end.
背景处理完全和皮肤处理一样,不做人工区域选择，仅使用魔术棒工具和50%不透明下面淡入淡出.收藏指正
2.⑵By searching the Bos_Taurus_WGS_Trace database with BLASTN program, we got many Contigs for RAPD marker S139-921. Using DNAMAN 5.0 software, we jointed these Contigs and extended the RAPD fragment S139-921 from 921 bp to 2141 bp in two orientations. The left end was extended 562 bp, and the right end was extended 658 bp.
初步推测该标记对应奶牛基因组中的一个LINE重复元件。 ⑵ 用BLASTN程序在Bos_Taurus_WGS_Trace数据库中搜索RAPD 标记S139-921片段的重叠群序列(Contigs),然后用DNAMAN 5.0软件进行拼接,使RAPD标记S139-921向右端延伸了658 bp,向左端延伸了562 bp,得到一个包含整个RAPD标记片断(921 bp)的序列,全长2141 bp。收藏指正
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