enzyme fractionation
1.The value of apparent K_M for CTP was 2×10~(-2)M. Attempts to prepare the enzyme in soluble form, suitable for fractionation by conventional methods, have proved unsuc cessful.
2.Radioactive tracing and electron microscope were used to evaluate the change of amino acid uptake, enzyme protein synthesis in acinar cell, subcellular fractionation, the quantitative analysis of mean zymogen granule number and mean zymogen granule area after injection L 3H phenylalanine 30,60,120 1nd 180 min on the 7 th day.
3.The arylamidase (E. 0. 3.4.11.2. AAD) of mouse kidney was purified by using Triton X-100 and trypsin treatment to solubilize the enzyme, and further combined with the use of ammonium sulfate fractionation and several types of column chroma-tography.
